When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature.
I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.
I didn't read the entire document in depth, but perhaps it has been shown elsewhere that first year GPA is a good quality predictor of GPA in subsequent years.
Yes and no. I enjoy getting personal tasks done in the morning before work, especially those that require a sharp mind. Like you mentioned, I feel worn out mentally and the end of the day, though I find I'm happier and more productive overall if I save a few sharp-minded hours in the early morning for myself.
People choose to be tested for Huntington disease, including many young adults who have recently turned 18 (in the USA). While, like yourself, I don't know what I would personally chose to do if faced with this situation, the availability of a specific test with a high NPV and low FP rate would be beneficial to have.
The only solution I can think of is for myself to buy a lifted truck or other similar unnecessarily large vehicle. I haven't done this, of course, but if I did I'd then be part of the problem but at least my eye line would be above that of the majority of other drivers. We have a sedan and a small SUV - I don't drive the sedan at night ever because of the headlight problem. My eyes hurt just thinking about this.
Mammalian cells in research labs are grown in media that is composed of 1) multiple powdered components that are readily obtainable through isolation from bacteria/yeast or synthesis and, 2) 10% FBS (fetal bovine serum). Until these cultured meat companies can come up with a suitable replacement for FBS, due to environmental, moral, cost, etc concerns, none of these solutions are terribly viable. I personally wouldn't want to eat (or pay for) a tiger streak that required liters of FBS to grow.
> I personally wouldn't want to eat (or pay for) a tiger streak that required liters of FBS to grow.
Since FBS is collected from a bovine fetus at slaughter houses, I'm not sure that anyone who currently eats meat and dairy should have an ethical problem with this. You're already paying for the animals to be slaughtered.
I assume that if this is a serum that comes only from bovine fetuses, more cows are slaughtered per pound of cultured meat than per pound of natural-grown meat. That would make this much less sustainable.
FSB is a byproduct of existing slaughter, not a special process which kills extra aninals. If you're opposed to the use of FSB because you thinks it's immoral to kill the animals, you should be equally opposed to the meat and dairy industries as they are today. Both are responsible for the death of animals and often fetuses.
There is a risk that the FSB collection process will allow the fetus to gain consciousness before death, but those paying into the meat and dairy industries are already comfortable with killing conscious, sentient animals.
It seems that you are thinking a little too short-term here. We have been culturing cells for >50 years. If this is a necessary ingredient, it may be a lot harder to substitute than you might think, which relegates lab-grown meat to staying a niche product. You will not have a viable, scalable meat replacement if your replacement needs to slaughter more cows per kilogram than meat.
As long as the amount of cultured meat is _relatively_ small, it's sustainable; FBS is essentially a byproduct, and no-one is slaughtering extra cows just to produce it.
If the cow was going to be slaughtered anyway, and you still get the meat, what does it matter if you get less serum per cow than meat per cow? Shouldn't we be glad we're getting more utility per cow slaughtered?
I'm wondering how they plan to get decent content. I just searched two of my interests, climbing and canoeing, and only a handful of uninteresting videos popped up. Are there specific topics that have engaging communities on the site?
Just like YouTube most content is crap. Maybe the bigger problem is that searching on any one instance will only search a small subset of the content available.
But it is mostly a discovery problem. Once you have found a channel that you like it is easy to follow via RSS or ActivityPub. I would love to see discovery services built on top of PeerTube, it seems natural to separate the viewing infrastructure from the recommendation service.
Quality is a function of effort. Effort has a cost. The typical cost for high quality is high effort.
A content creator producing quality content has spent a lot of money in order to produce the high quality content. How much? Estimates from YouTube creators who are currently in the top creators get us numbers like $25,000-$1,000,000 per video. If they do not reliably make more than that much from trying to produce at that quality, they cannot survive while doing so.
So a larger problem than content discovery is that by default people producing quality content will die.
There is a huge difference between "there is lots of crap content to wade through to get to the good content" and "I can't find content that isn't crap". My foray into Peertube over the years is that the latter is much closer than the former.
Well, most of everything in the Universe is “crap.” But, to each their own, and I find YouTube to be a treasure trove of educational content, among other things.
It's different. Most people on Twitter are there to promote their ideas and themselves, not get paid. So free syndication is fine for them. Most popular/quality YouTube content is there because the creator wants to get paid, not to spread their ideas.
… I mean, I think 99.99% of people are on twitter to have fun, not promote themselves. (Also some of the remainder are on twitter specifically to moan about bad customer service; it’s surprising how many accounts you we apparently used solely for this).
Similarly, most people on YouTube are not expecting to strike it big with videos of their cat, or their hobby project, or whatever.
Much of the content I watch on YouTube consists of obscure music videos where nobody is getting paid (or maybe they have a Patreon), but having someone else host your videos for free is still a pretty good deal.
That's like asking how Signal plans on getting nice people to talk to. Peertube is just a self-hosted platform that allows for bandwidth-efficient video sharing, it's up to the single instance owners to care about publicizing their stuff.
It's open source. You could put Google Adsense API calls in every nook and cranny of the platform if you like. Or any other ad provider, for the matter
The point of what? The point of PeerTube is to leverage the p2p nature of torrents to allow people to host video-sharing platforms for a fraction of the cost. If you want to monetize your instance then why not?
I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.